Comparative evaluation of the conventional tube test and column agglutination technology for ABO antibody titration in healthy individuals: a report from India

Abstract Determination of accurate anti-A/-B titers is important for treatment selection in ABO-incompatible stem cell and solid-organ transplants. The standard method ABO antibody titration the conventional tube test (CTT). Dithiothreitol (DTT) commonly used to inactivate IgM component. aim this study was compare six different methods observe effectiveness DTT on estimation. A total 90 healthy voluntary blood donors were enrolled study, including 30 each groups A, B, O. Antibody titrations performed tested using CTT-immediate spin (IS), CTT-antihuman globulin (AHG) with without DTT, column agglutination technology (CAT)-IS, CAT-AHG methods. Bead-CAT used, positive cutoff value set 1+ determine endpoint titer. median values by IS found be higher than those AHG CTT CAT among group B individuals, whereas no statistically significant differences observed from O individuals titers, estimated method. Although there correlation between titer results obtained all groups, testing showed poor agreement pretreatment (kappa 0.11 0.20, respectively). Moderate CTT-IS CAT-IS 0.46). Median reduced use samples. Significant interpretability uniform approach selecting highly recommended, plasma neutralize activity should considered obtain precise IgG titers. Immunohematology 2021;37:25–32 .